Prevalence of Plasmodium falciparum antimalarial drug resistance genes in Southeastern Gabon from 2011 to 2014
Identifieur interne : 000078 ( France/Analysis ); précédent : 000077; suivant : 000079Prevalence of Plasmodium falciparum antimalarial drug resistance genes in Southeastern Gabon from 2011 to 2014
Auteurs : Dominique Fatima Voumbo-Matoumona [France] ; Lady Charlene Kouna ; Marylin Madamet [France] ; Sydney Maghendji-Nzondo ; Bruno Pradines [France] ; Jean Bernard Lekana-DoukiSource :
- Infection and Drug Resistance [ 1178-6973 ] ; 2018.
Descripteurs français
- Wicri :
- geographic : Gabon.
English descriptors
Abstract
Purpose: The introduction of artemisinin-based combination therapies (ACTs) in treating uncomplicated malaria and sulfadoxine-pyrimethamine (SP) as intermittent preventive treatment during pregnancy drastically decreased the burden of malarial disease around the world. However, ACTs are known to select for drug resistance markers. In Gabon, artemether-lumefantrine induced an increase in the prevalence of N86-Pfmdr1, which is associated with treatment failure. However, little data are available regarding resistance markers in Southeastern Gabon. This study aimed to evaluate the evolution of resistance haplotypes in the Pfcrt, Pfdhps, Pfdhfr, and PfK13 genes from 2011 to 2014 in Southeastern Gabon. Methods: A total of 233 Plasmodium falciparum DNA samples were collected from febrile pediatric patients in South Gabon: Franceville, an urban area; Koulamoutou, a semi-urban area; and Lastourville, a rural area. Pfcrt, Pfdhps, Pfdhfr, and the propeller domain of PfK13 were sequenced for all isolates. Results: The overall prevalence (3.7%-11.5%) of the wild-type haplotype Pfcrt 72-76 CVMNK was not significantly different between 2011 and 2014 in Southeast Gabon. For Pfdhfr (codons 51, 59, 108, 164), the IRNI triple-mutant haplotype was the most prevalent (>89.0%). The ICNI and NCNI mutant haplotypes and the NCSI wild-type haplotype showed a minor prevalence. There were no differences in the distributions of these haplotypes across the 4 years and the three study sites. For Pfdhps, the AAKAA and SGKAA mutant haplotypes and the SAKAA wild-type haplotype were similarly present in the three areas during the study period. The AGKAA double mutant was first observed in 2013 in Franceville and in 2014 in Koulamoutou and Lastourville. Interestingly, only the A578S mutation (0.4%) and two new A494V (0.4%) and V504A (0.9%) mutations were found in PfK13. Conclusion: Despite the withdrawal of chloroquine, the frequency of the resistant allele 76T remained high in the south of Gabon. Moreover, a high level of resistant haplotypes against IPTp-SP was found.
Url:
DOI: 10.2147/IDR.S160164
Affiliations:
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Hal:hal-01924151Le document en format XML
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Charlene" last="Kouna">Lady Charlene Kouna</name></author><author><name sortKey="Madamet, Marylin" sort="Madamet, Marylin" uniqKey="Madamet M" first="Marylin" last="Madamet">Marylin Madamet</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-527045" status="VALID"> <idno type="RNSR">201822675Y</idno> <orgName>Vecteurs - Infections tropicales et méditerranéennes</orgName> <orgName type="acronym">VITROME</orgName> <date type="start">2018-01-01</date> <desc> <address> <addrLine>IHU Méditerranée Infection19-21 Bd Jean Moulin13005 Marseille</addrLine> <country key="FR"></country> </address> </desc> <listRelation> <relation name="UMR_D 257" active="#struct-67872" type="direct"></relation> <relation name="UMR MD4" active="#struct-198056" type="direct"></relation> <relation active="#struct-507058" type="direct"></relation> </listRelation> <tutelles><tutelle name="UMR_D 257" active="#struct-67872" type="direct"><org type="institution" xml:id="struct-67872" 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<orgName>Institut de Recherche Biomédicale des Armées</orgName> <orgName type="acronym">IRBA</orgName> <desc> <address> <addrLine>Brétigny-sur-Orge</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.defense.gouv.fr/sante/notre-expertise/recherche-biomedicale/recherche-biomedicale</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Marseille</settlement><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName><orgName type="university">Université d'Aix-Marseille</orgName></affiliation></author><author><name sortKey="Maghendji Nzondo, Sydney" sort="Maghendji Nzondo, Sydney" uniqKey="Maghendji Nzondo S" first="Sydney" last="Maghendji-Nzondo">Sydney Maghendji-Nzondo</name></author><author><name sortKey="Pradines, Bruno" sort="Pradines, Bruno" uniqKey="Pradines B" first="Bruno" last="Pradines">Bruno Pradines</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" 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<ref type="url">http://www.ird.fr/</ref> </desc> </org></tutelle><tutelle name="UMR MD4" active="#struct-198056" type="direct"><org type="institution" xml:id="struct-198056" status="VALID"> <idno type="IdRef">15863621X</idno> <idno type="ISNI">0000 0001 2176 4817</idno> <orgName>Aix Marseille Université</orgName> <orgName type="acronym">AMU</orgName> <date type="start">2012-01-01</date> <desc> <address> <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine> <country key="FR"></country> </address> <ref type="url">http://www.univ-amu.fr/</ref> </desc> </org></tutelle><tutelle active="#struct-507058" type="direct"><org type="institution" xml:id="struct-507058" status="VALID"> <orgName>Institut de Recherche Biomédicale des Armées</orgName> <orgName type="acronym">IRBA</orgName> <desc> <address> <addrLine>Brétigny-sur-Orge</addrLine> <country key="FR"></country> </address> <ref type="url">https://www.defense.gouv.fr/sante/notre-expertise/recherche-biomedicale/recherche-biomedicale</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>France</country><placeName><settlement type="city">Marseille</settlement><region type="region" nuts="2">Provence-Alpes-Côte d'Azur</region></placeName><orgName type="university">Université d'Aix-Marseille</orgName></affiliation></author><author><name sortKey="Lekana Douki, Jean Bernard" sort="Lekana Douki, Jean Bernard" uniqKey="Lekana Douki J" first="Jean Bernard" last="Lekana-Douki">Jean Bernard Lekana-Douki</name></author></analytic><idno type="DOI">10.2147/IDR.S160164</idno><series><title level="j">Infection and Drug Resistance</title><idno type="ISSN">1178-6973</idno><imprint><date type="datePub">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="mix" xml:lang="en"><term>Gabon</term><term>PfK13</term><term>Pfcrt</term><term>Pfdhfr/Pfdhps</term><term>Plasmodium falciparum</term><term>antimalarial drug resistance</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Gabon</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"> <p>Purpose: The introduction of artemisinin-based combination therapies (ACTs) in treating uncomplicated malaria and sulfadoxine-pyrimethamine (SP) as intermittent preventive treatment during pregnancy drastically decreased the burden of malarial disease around the world. However, ACTs are known to select for drug resistance markers. In Gabon, artemether-lumefantrine induced an increase in the prevalence of N86-Pfmdr1, which is associated with treatment failure. However, little data are available regarding resistance markers in Southeastern Gabon. This study aimed to evaluate the evolution of resistance haplotypes in the Pfcrt, Pfdhps, Pfdhfr, and PfK13 genes from 2011 to 2014 in Southeastern Gabon. Methods: A total of 233 Plasmodium falciparum DNA samples were collected from febrile pediatric patients in South Gabon: Franceville, an urban area; Koulamoutou, a semi-urban area; and Lastourville, a rural area. Pfcrt, Pfdhps, Pfdhfr, and the propeller domain of PfK13 were sequenced for all isolates. Results: The overall prevalence (3.7%-11.5%) of the wild-type haplotype Pfcrt 72-76 CVMNK was not significantly different between 2011 and 2014 in Southeast Gabon. For Pfdhfr (codons 51, 59, 108, 164), the IRNI triple-mutant haplotype was the most prevalent (>89.0%). The ICNI and NCNI mutant haplotypes and the NCSI wild-type haplotype showed a minor prevalence. There were no differences in the distributions of these haplotypes across the 4 years and the three study sites. For Pfdhps, the AAKAA and SGKAA mutant haplotypes and the SAKAA wild-type haplotype were similarly present in the three areas during the study period. The AGKAA double mutant was first observed in 2013 in Franceville and in 2014 in Koulamoutou and Lastourville. Interestingly, only the A578S mutation (0.4%) and two new A494V (0.4%) and V504A (0.9%) mutations were found in PfK13. Conclusion: Despite the withdrawal of chloroquine, the frequency of the resistant allele 76T remained high in the south of Gabon. Moreover, a high level of resistant haplotypes against IPTp-SP was found.</p> </div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Provence-Alpes-Côte d'Azur</li></region><settlement><li>Marseille</li></settlement><orgName><li>Université d'Aix-Marseille</li></orgName></list><tree><noCountry><name sortKey="Kouna, Lady Charlene" sort="Kouna, Lady Charlene" uniqKey="Kouna L" first="Lady Charlene" last="Kouna">Lady Charlene Kouna</name><name sortKey="Lekana Douki, Jean Bernard" sort="Lekana Douki, Jean Bernard" uniqKey="Lekana Douki J" first="Jean Bernard" last="Lekana-Douki">Jean Bernard Lekana-Douki</name><name sortKey="Maghendji Nzondo, Sydney" sort="Maghendji Nzondo, Sydney" uniqKey="Maghendji Nzondo S" first="Sydney" last="Maghendji-Nzondo">Sydney Maghendji-Nzondo</name></noCountry><country name="France"><noRegion><name sortKey="Voumbo Matoumona, Dominique Fatima" sort="Voumbo Matoumona, Dominique Fatima" uniqKey="Voumbo Matoumona D" first="Dominique Fatima" last="Voumbo-Matoumona">Dominique Fatima Voumbo-Matoumona</name></noRegion><name sortKey="Madamet, Marylin" sort="Madamet, Marylin" uniqKey="Madamet M" first="Marylin" last="Madamet">Marylin Madamet</name><name sortKey="Pradines, Bruno" sort="Pradines, Bruno" uniqKey="Pradines B" first="Bruno" last="Pradines">Bruno Pradines</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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